Beginner Gating Strategies to Start Analyzing Your Flow Cytometry Data
No matter what cell type you’re interested in exploring with flow cytometry, the majority of analyses start with the same basic gating strategy.
No matter what cell type you’re interested in exploring with flow cytometry, the majority of analyses start with the same basic gating strategy.
In Case Study 1 Part 1 I use FCS files publicly available from FlowRepository to go through a detailed analysis of a dataset, from data cleaning to sophisticated cluster-based data extraction.
Flow cytometry dot plots need to be optimized for grants and publications, but the way you choose to display the plots can affect the clarity and purpose of the data. Finding the best resolution for your flow cytometry plots ensures accuracy and meaningfulness of the data.