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Marissa Fahlberg, PhD
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Flow Cytometry

Tutorial: Make a tSNE Plot in FlowJo with Flow Cytometry Data

December 1, 2019November 29, 2019 by mfahlberg824

This tutorial describes how to use tSNE to analyze flow cytometry data in FlowJo, and also teaches advanced tSNE visualizations.

Categories Flow Cytometry, tSNE

Beginner Gating Strategies to Start Analyzing Your Flow Cytometry Data

December 9, 2019October 31, 2019 by mfahlberg824

No matter what cell type you’re interested in exploring with flow cytometry, the majority of analyses start with the same basic gating strategy.

Categories Dot Plots, Flow Cytometry

A Basic Overview of Using t-SNE to Analyze Flow Cytometry Data

December 5, 2019September 29, 2019 by mfahlberg824

t-SNE is an algorithm used for arranging high-dimensional data points in a two-dimensional space so that events which are highly related by many variables are most likely to aggregate closely together.

Categories Flow Cytometry, tSNE

How Fluorochromes are Used in Flow Cytometry

November 18, 2019May 20, 2019 by mfahlberg824

Fluorochromes are critical components of flow cytometry experiments and rely heavily on the principles of fluorescence in order to function. In this post I’ll explain how fluorescence works, and then dive into fluorochromes and flow cytometry.

Categories Flow Cytometry, Fluorescence

Case Study 1 Part 2: Normalization and Preliminary Analyses

May 20, 2019May 12, 2019 by mfahlberg824

In Case Study 1 Part 2, I explain the process of data normalization, or aligning peaks of data to make gating quicker and easier, as well as make the data suitable for clustering analyses.

Categories Flow Cytometry

Case Study 1 Part 1: Introduction, Data Cleaning and Pre-processing

November 18, 2019April 11, 2019 by mfahlberg824

In Case Study 1 Part 1 I use FCS files publicly available from FlowRepository to go through a detailed analysis of a dataset, from data cleaning to sophisticated cluster-based data extraction.

Categories Dot Plots, Flow Cytometry
flow-cytometry-dot-plot-analysis-low-resolution

Resolution of Flow Cytometry Data – What it Means and Why it Matters for Publications

September 29, 2019March 31, 2019 by mfahlberg824

Flow cytometry dot plots need to be optimized for grants and publications, but the way you choose to display the plots can affect the clarity and purpose of the data. Finding the best resolution for your flow cytometry plots ensures accuracy and meaningfulness of the data.

Categories Dot Plots, Flow Cytometry
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