t-SNE is an algorithm used for arranging high-dimensional data points in a two-dimensional space so that events which are highly related by many variables are most likely to aggregate closely together.
Fluorochromes are critical components of flow cytometry experiments and rely heavily on the principles of fluorescence in order to function. In this post I’ll explain how fluorescence works, and then dive into fluorochromes and flow cytometry.
Flow cytometry dot plots need to be optimized for grants and publications, but the way you choose to display the plots can affect the clarity and purpose of the data. Finding the best resolution for your flow cytometry plots ensures accuracy and meaningfulness of the data.